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F4042
Sigma
Monoclonal ANTI-FLAG® M5 antibody produced in mouse
clone M5, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD00282246 |
Description
| Application | Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli. |
| Other Notes | To view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature. |
| Physical form | Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v) |
| Legal Information | ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| antibody form | purified immunoglobulin |
| clone | M5 (monoclonal) |
| form | buffered aqueous solution |
| application(s) | immunoblotting (chemiluminescent): 10 μg/mL |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Related product | F1804, Monoclonal ANTI-FLAG® M2 antibody produced in mouse |
